Analytical and Physical Seminar - Iain Campuzano

The Analytical and Physical Seminar Series presents: Iain Campuzano 

Amgen

Host: Jenny Brodbelt

Title: Mass Spectrometry in Biopharma: From Fundamental Gas Phase Structure to Therapeutic Project Support

Location: WEL 2.122

Mass spectrometry (MS) plays a key role throughout all stages of drug development and is now as ubiquitous as other analytical techniques such as surface plasmon resonance, nuclear magnetic resonance and supercritical fluid chromatography, among many others.  Mass spectrometry within biopharma has contributed to numerous key applications and therapeutic developments, too numerous to discuss specifically herein.  

However, I will highlight a personal journey within biopharma that has progressed through many interesting and fundamental small molecule and protein gas-phase ion structure studies. For example, I will discuss the first T-Wave small molecule calibration protocol and the associated N₂-based Trajectory Method optimization and ionic surface charge distribution and its impact on the ions mobility.  I will also discuss the observed ionic structural collapse for mAbs and MsAbs. I will also discuss the use of ion mobility instrumentation, RF-confining drift cell, time-of-flight and Fourier transform ion cyclotron mass spectrometers for high m/z analysis, within biopharma.

Finally, I will also discuss key therapeutic areas where mass spectrometry has led to first in class therapeutics. For example, high throughput mass spectrometry was a key analytical method during the discovery and screening stages of LUMAKRAS, the first small molecule therapeutic that covalently targets the mutated oncogene KRAS^G12C. Also, I’ll present how both denaturing and native-MS mass spectrometry is being used within Amgen to support membrane protein characterization.